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Beside the standard RBC-Ready Gene MNS and RBC-Ready Gene KKD test systems we offer the RBC-Ready Gene KELplus kit for detection of further Kell alleles, as well as the RBC-Ready Gene JKplusFY for detection of further Kidd alleles in combination 2012-04-26 Molecular PCR typing allowed for precise antigen determination in all the patients. Comparing both methods we found PCR-SSP HLA typing method very useful in routine HLA-DR determination, especially valuable in patients, in whom some problems in serological testing are expected. PMID: 9869840 [Indexed for MEDLINE] Publication Types: Comparative Study Application: Ready-to-use PCR-SSP typing kits allow the determination of common, rare, or weak alleles of the ABO blood group, Rhesus, and Kell/Kidd/Duffy systems as well as alleles of the human platelet antigens. Results: The investigations showed clear-cut results in accordance with serology or molecular genetic pretyping. This video shows two critical steps in the process of KIR-gene typing by PCR-SSP:1-How to prepare 24 ready-to-use typing sets2-The rapid loading of six typin Most molecular HLA typing methods are based on the group-specific amplification by PCR where the PCR-SSP technique is widely used to detect HLA-B* 27 .

CONCLUSION: PCR-SSP is a helpful supplementary technique for resolving most of the common problems caused by discrepant or doubtful serologic results, and it is an easy-to-handle robust method. Questionable cases in donor, recipient, and patient typing can be examined with acceptable cost. T he molecular genetic basis of almost all blood INTRODUCTION Sequence Specific Primers Pcr Ssp Typing Kit, supplied by Pel-Freez, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more PCR-SSP is a helpful supplementary technique for resolving most of the common problems caused by discrepant or doubtful serologic results, and it is an easy-to-handle robust method. Questionable cases in donor, recipient, and patient typing can be examined with acceptable cost.

The Dynal SSP products consist of Current DNA-based methods that are in use for HLA typing are polymerase chain reaction-sequence-specific priming (PCR-SSP).

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av I huvudet på en ST-läkare — Type I Interferon and Clinical Phenotype in Idiopathic. Inflammatory HLA-A, -C and HLA-DRB1 allele typing was performed by SSP-PCR in. 77 families with PCR and gene micro arrays. The studies are RE, Santosham M. Effectiveness of Haemophilus influenzae Type B Conjugate Vaccin on.

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Troubleshooting This chapter describes a polymerase chain reaction sequence-specific primer (PCR-SSP) typing system that is applicable to the identification of all HLA class I and class II alleles, as well as alleles from non-HLA loci (10, 11) . PCR-SSP is also variably known as allele-specific PCR or amplification refractory mutation system (ARMS).

Questionable cases in donor, recipient, and patient typing can be examined with acceptable cost. PCR-SSP overcame the obstacles presented by serological HLA typing: limited, polyclonal antisera, the need to live cells, and the inability to ‘capture’ raw data for future reference. However, PCR-SSP requires gel electrophoresis for the separation of amplified DNA fragments, and ethidium bromide (a suspected carcinogen) and UV transillumination present significant health and safety concerns. 2 3 Rheumatology International Clinical and Experimental Investigations ISSN 0172-8172 Volume 33 Number 4 Rheumatol Int HLA-DRβ1*04 typing by simple in- house PCR-SSP technique for rheumatoid arthritis patients Nested PCR-SSP is a two-step approach to HLA typing whereby the region of interest is amplified in the first step and this amplicon is used instead of genomic DNA for the second, sequence-specific amplifications, using primers which are internal to the first pair of amplification primers. 2021-03-14 · PCR with sequence speciﬁc primers (PCR-SSP) One commonly applied approach is to use panels of sequence speciﬁc DNA primers which amplify particular HLA alleles or allele groups. PCR-SSP is a very rapid test that can be performed in 3 – 4 hours and is commonly used for typing deceased organ donors when speed is an important consideration.
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Comparison of results obtained by both methods revealed no discrepancies in 5 patients, in 12 patients the PCR-SSP typing showed additional DR antigens or splits of antigens. HLA typing by sequence-specific primers (PCR-SSP) Amplification with sequence-specific primers yields only a product if the target sequences are present in the DNA sample (compare lane 7 and 8 with the figure) In total 16 primers are used for the analysis of HLA-DR4 allele.

Red Blood Cell-Ready Gene is inno-train's product line for analysis of erythrocyte blood groups based on the SSP-PCR method. Therefore these diagnostic systems represent the ideal complement to serolgoical blood group typing. The modular kit design offers an extensive choice for a gradual typing system depending on your problem.
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Discrepant HLA typing results were detected in 6 of 33 samples (18.2%) analyzed by two different PCR-SSO techniques and one PCR-SSP About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features Press Copyright Contact us Creators In typing by PCR amplification with sequence-specific primers (PCR-SSP), typing specificity is part of the amplification step, which makes the technique almost as fast as serological tissue typing. BAGene SSP - reliable molecular blood group typing for few samples or for specific detection tests is the PCR-SSP technique. The kits contain a validated and IVD-CE-certified Taq polymerase to improve your result. typing.

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html. Create Close. One-step HLA determination to allelic resolution: PCR-SSP HLA-A and -Cw high resolution typing reaction (PCR–SSP) DNA typing for the Rh e antigen in four. independent patients. Two could be resolved by family tree. Correspondence. Genotyping topics discussed include well-established RFLP analysis and polymerase chain reaction applications of PCR-SSO, PCR-RFLP, PCR-SSP, and PCR (PCR-.

Rapid DNA typing for HLA-C using sequence-specific primers (PCR-SSP): Identification of serological and non-serologically defined HLA-C alleles including several new alleles Article Feb 1994 PCR-SSP typing and subsequent nonradioac tive sequence-based typing for HLA DRB1/3/4/5, DQB1, and DPB1 allel es. Tissue Antigens 1994 Nov;44(5):275-84.