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On-chip ultrasonic sample preparation for cell based assays

cell transplantation has developed as a standard treatment for many cancers besides development of several novel therapies for castrate-resistant prostate cancer, ultrasonic wave based microfluidic techniques (acoustophoresis) to enable. Umeå universitet, får 820 000 kronor. Projekt: Apheresis-acoustophoresis to improve cancer diagnosis in prostate cancer (APACA-PC). I have a broad skill set and I have so far used it mainly in cancer related applications. Decomplexing biofluids using microchip based acoustophoresis. Integrated whole blood acoustophoresis and homogeneous nucleic Detta öppnar såklart upp möjligheterna att via blodprov testas för cancer. Post-transcriptional control of stem and cancer cell fate : new roles for an old cell preparations by acoustophoresis / Franziska Olm. 2020; BokAvhandling.

Materials and methods Microbead suspensions For nonfixed, viable cells, tumor cell recovery ranged from 72.5% to 93.9% with purity ranging from 79.6% to 99.7%. These data contribute proof-in-principle that label-free microfluidic acoustophoresis can be used to enrich both viable and fixed cancer cells from WBCs with very high recovery and purity. design and use of acoustophoresis micro-devices for long-term cell handling, and confirm that standing-wave ultrasound is a suitable cell manipulation technology for clinical applications even at high pressure amplitudes. Materials and Methods Cell line, culture and labeling. In this work we used the human lung cancer cell line A549 acoustophoresis (uncountable) acoustic levitation ; a method for suspending matter in a medium by using acoustic radiation pressure from intense sound waves in the medium 2015 November 12, “Cancer Cell Analyses at the Single Cell-Level Using Electroactive Microwell Array Device”, in PLOS ONE ‎ [1] , DOI : 10.1371/journal.pone.0139980 : Measuring density and compressibility of white blood cells and prostate cancer cells by microchannel acoustophoresis.

Second part of the thesis focuses on acoustophoresis. In (paper 5), a multifunctional acoustic microdevice was developed for isolation of cancer cells from red blood cells with a separation efficiency of 92.4% and trapping efficiency of 93%.

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Surface-marker-based detection of CTC predicts recurrence and survival in colorectal, breast, and prostate cancer. acoustophoresis based separation reports a cancer cell recovery up to 87% (i.e.

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External funding 33.6 MSEK PI: Prof. Thomas Laurell, Lund University Co-PI: Dr. David Ulmert, Prof. David Erlinge, Prof. Hans Lilja, Prof.

According to the American Cancer Society, a Lung cancer is a serious illness which none of us wish to face. Here we look at some of the key symptoms of this disease to watch out for. We also explore how it is diagnosed and the many treatment options now available should you be unfort Lung cancer is a leading type of cancer — and a leading killer — in the United States every year.
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Materials and methods Microbead suspensions For nonfixed, viable cells, tumor cell recovery ranged from 72.5% to 93.9% with purity ranging from 79.6% to 99.7%.

Cell viability was determined after acoustophoresis (0, 10 and 20 V pp), for four prostate cancer cell lines: DU 145 (a), PC3 (b), LNCaP (c) and VCaP (d).
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‪Cecilia Magnusson‬ - ‪Google Scholar‬

acoustophoresis (acoustic manipulation) while the passive method is based on inertial microfluidics (hydrodynamic manipulation). In paper I, acoustic capillary-based cavity resonator was used to study aggregation of silica and polystyrene particles. We found that silica particles show faster aggregation Andreas Josefsson/Stina Rudolfsson, Kirurgi och perioperativa vetenskaper, Umeå universitet, fick 820 000 kronor. Projekt: Apheresis-acoustophoresis to improve cancer diagnosis in prostate cancer (APACA-PC). Anna Lantz, lnst för Medicinsk Epidemiologi och Biostatistik, Karolinska Institutet, fick 388 784 kronor. Neither did acoustophoresis affect cell proliferation or the ability of cells to be recultured for several passages ( Figure 4B).

List of publications

acoustophoresis based separation reports a cancer cell recovery up to 87% (i.e. 1 3% loss) on clinical scale sample volumes [46] , only accounting for losses from spiking 50 cancer cells in 1 mL, monitoring and characterizing of metastatic cancer. KEYWORDS: Micro-PIV, Ultrasound Resonances, Acoustophoresis, Cell Handling INTRODUCTION Microchannel acoustophoresis is a rapidly expanding research ﬁeld allowing gentle and efﬁcient manipulation of cells and other biological particles [1, 2]. The larger cancer cells are acoustophoretically driven into the center stream by the radiation force, whereas white and red blood cells do not migrate as far in the acoustic field and thus exit through the side outlets.

15 Indeed, Yang and colleagues demonstrated that microfluidic acoustophoresis could be used to effectively concentrate viable breast cancer cells (MCF7). 16 Osmotically induced cell death reduces the cell size and when mixed with larger, viable cells, the authors successfully concentrated the viable cells based on Cancer cells display acoustic properties enabling acoustophoretic separation from white blood cells (WBCs) with 2–3 log suppression of the WBC background. However, a subset of WBCs has overlapping acoustic properties with cancer cells, which is why label-free acoustophoretic cancer cell isolation needs additional purification prior to analysis. Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis | Analytical Chemistry Circulating tumor cells (CTC) are shed in peripheral blood at advanced metastatic stages of solid cancers. Surface-marker-based detection of CTC predicts recurrence and survival in colorectal, breast, and prostate cancer. Acoustophoresis is a non-contact and label-free mode of manipulating particles and cell populations and allows for implementation of several separation modes [ 2 ]. The technology is currently finding increased applications in bioanalytical and clinical applications of cell handling and manipulation.